Analysis Note

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Application

Acts specifically on single-stranded DNA degrading it processively in the 3′- to 5′-direction, producing 5′-mononucleotides.

Biochem/physiol Actions

Exonuclease I (EXO1) maintains genomic stability by participating in DNA mismatch repair system, DNA recombination, DNA replication and DNA double-stranded break repair in eukaryotes. It also modulates meiosis, immunoglobulin maturation, and micro-mediated end-joining. It functions on DNA with blunt, nicked, gapped and 3′-overhangs. It also possesses endonuclease action and removes 5′-single-stranded flaps that protrudes from a double stranded DNA. Mutation in EXO1 gene results in increased susceptibility to develop cancer.

Features and Benefits

Eliminating residual single-stranded DNA containing a 3′-terminus. Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA. Measuring DNA helicase activity.

General description

Exonuclease I.

The EXO1 (exonuclease I) gene is mapped to human chromosome 1q43 and codes for a 5′→3′ exonuclease of 846 amino acids. It belongs to the 5′-nuclease superfamily.